Utilizing TPGS for Optimizing Quercetin Nanoemulsion for Colon Cancer Cells Inhibition

Background: Colorectal cancer is one of the most challenging cancers to treat. Exploring novel therapeutic strategies necessary overcome drug resistance and improve patient outcomes. Quercetin (QR) a polyphenolic lipophilic compound that was chosen due its colorectal anticancer activity. Nanoparticles could therapy via tumor targeting by utilizing D-tocopheryl polyethylene glycol succinate (vitamin-E TPGS) as surfactant in nanoemulsion preparation, which considered an efficient delivery system for enhancing antineoplastic agents. Thus, this study aims develop optimize QR-loaded nanoemulsions (NE) using TPGS enhance QR antitumor Method: The NE prepared self-assembly technique oils according maximum solubility surfactant. QR-NE evaluated particle morphology pH. entrapment efficiency vitro release rate were determined from selected then we measured stability. activity best-selected formula studied on HT-29 HCT-116 cell lines. Results: Oleic acid prepare it has best solubility. NE, had particles size < 200 nm, > 80%, pH 3.688 + 0.102 optimal formula. It physically stable enhanced (84.52 ± 0.71%) compared free drug. QR-NPs significantly improved cellular killing colon lines (lower IC50, two folds more than drug). Conclusion: be promising improving